Activated Protein C Resistance : Molecular Mechanisms Based on Studies Using Purified G 1 n 5 O 6 - Factor
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چکیده
Gln=-factor V (W) was purified from plasma of an individual homozygous for an Arg506Gln mutation in FV that is associated with activated protein C (APC) resistance. Purified GlnW-FV, as well as Glnm-FVa generated by either th ombin or FXa, conveyed APC resistance to FV-deficient plasma in coagulation assays. Clotting assay studies also suggested that APC resistance does not involve any abnormality in FVAPC-cofactor activity. In purified reaction mixtures, GlnmFVa in comparison to normal N a showed reduced susceptibility to APC, because it was inactivated -10-fold slower than normal ArgW-FVa. Itwas previously reported that inactivation of normal FVa by APC involves an initial cleavage at Argm followed by phospholipid-dependent cleavage at
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تاریخ انتشار 2000